This study investigates the analgesic activity of Jeruju leaf infusion (Acanthus ilicifolius L.) on male white mice (Mus musculus). Acanthus ilicifolius, commonly known as Jeruju, has been traditionally employed for its potential therapeutic properties, including pain relief. The experiment involved administering varying concentrations of Jeruju leaf infusion to the experimental group, while the control group received a placebo. The analgesic effect was assessed using standard pain-inducing stimuli, and the responses were recorded and analyzed. Preliminary findings suggest a dose-dependent response in the experimental group, indicating a potential analgesic effect of Jeruju leaf infusion. The results were statistically significant, demonstrating a reduction in pain responses compared to the control group. Further investigation into the underlying mechanisms and active compounds responsible for the observed analgesic activity is warranted. This study contributes to the growing body of research exploring natural remedies for pain management. The potential analgesic properties of Jeruju leaf infusion highlight its promising role in traditional medicine. Future studies could delve deeper into the pharmacological aspects, dosage optimization, and long-term effects of Acanthus ilicifolius L. in managing pain. The findings may have implications for the development of novel analgesic agents derived from natural sources, offering alternative approaches to pain relief with potential clinical applications.